While we haven't examined PneumaCult™ media to be used with murine epithelial cells, you'll find publications that report this application.
Evaluate preclinical exam compounds and biologics for their talents to modulate the immune system.
If applying Gentle Cell Dissociation Reagent (GCDR), the period of GCDR remedy must be optimized. Incubate organoids with GCDR for 8 -ten minutes at 37°C and use a P1000 pipette to Carefully pipette up and down, and visually inspect if organoids crack up.
If cells never dissociate very well with trituration, go with an extended ACCUTASE™ incubation, as many as twelve minutes. If monolayers will not dissociate immediately after twelve minutes of ACCUTASE™ incubation and trituration, the differentiation was very likely unsuccessful.
The STEMdiff™ system offers a standardized method for differentiating hPSCs into epithelial cells which can be later on cultured in 2nd or 3D formats according to the analysis desires.
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Observe: Carry on to antibody staining if carrying out Reside cell Examination immediately after this step. Alternatively, continue to fixable dye staining and PFA fixation.
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